DATF - The Database of Arabidopsis Transcription Factor
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  • ARF Family Introduction
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  • Binding Site of This Family
  • Download Sequences of This Family
          Genomic sequence       cds sequence       protein sequence
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    • List of ARF family(Total: 23 Loci; 34 Gene Models):

    LocusGene ModelsDescription
    AT1G19220AT1G19220.1 Symbol: ARF19 | transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related, contains Pfam profile: PF02309 AUX/IAA family | chr1:6628059-6632770 REVERSE | Aliases: ARF11, IAA22, T29M8.9, T29M8_9
    AT1G19850AT1G19850.1 Symbol: MP | transcription factor MONOPTEROS (MP) / auxin-responsive protein (IAA24) / auxin response factor 5 (ARF5), identical to transcription factor MONOPTEROS (MP/IAA24/ARF5) SP:P93024 from (Arabidopsis thaliana) | chr1:6886870-6891365 FORWARD | Aliases: ARF5, F6F9.10, F6F9_10, IAA24, MONOPTEROS, MP, TRANSCRIPTION FACTOR IAA24
    AT1G30330AT1G30330.1 AT1G30330.2 Symbol: ARF6 | similar to auxin-responsive factor (ARF8) [Arabidopsis thaliana] (TAIR:At5g37020.1); similar to auxin response factor 6b [Oryza sativa] (GB:BAB85915.1); similar to OSJNBb0004A17.5 [Oryza sativa (japonica cultivar-group)] (GB:XP_474307.1); similar to auxin response factor 4 [Cucumis sativus] (GB:BAD19064.1); similar to putative auxin response factor [Oryza sativa (japonica cultivar-group)] (GB:BAD45924.1); similar to putative auxin response transcription factor(ARF6) [Oryza sativa (japonica cultivar-group)] (GB:XP_464221.1); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340) | chr1:10685804-10690018 REVERSE | Aliases: ARF6, AUXIN RESPONSE FACTOR 6, T4K22.6, T4K22_6
    AT1G34170AT1G34170.1 AT1G34170.2 Symbol: ARF13 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g35540.1); similar to ARFT_ARATH Putative auxin response factor 20 (GB:Q9C7I9); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340) | chr1:12443525-12446742 REVERSE | Aliases: ARF13, AUXIN RESPONSE FACTOR 13, F12G12.1, F12G12_1
    AT1G34310AT1G34310.1 Symbol: ARF12 | transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related, contains Pfam profile: PF02309 AUX/IAA family | chr1:12508526-12511498 REVERSE | Aliases: ARF12, AUXIN RESPONSE FACTOR 12, F23M19.4, F23M19_4
    AT1G34390AT1G34390.1 Symbol: ARF22 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g35540.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g35240.1); similar to transcriptional factor B3 family protein [Arabidopsis thaliana] (TAIR:At1g35520.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g34410.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g34310.1); similar to ARFT_ARATH Putative auxin response factor 20 (GB:Q9C7I9); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340); contains InterPro domain AUX/IAA protein (InterPro:IPR003311) | chr1:12555983-12559060 FORWARD | Aliases: ARF22, AUXIN RESPONSE FACTOR 22, F7P12.6, F7P12_6
    AT1G34410AT1G34410.1 Symbol: ARF21 | transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related, contains Pfam profile: PF02309 AUX/IAA family | chr1:12577700-12580802 FORWARD | Aliases: ARF21, AUXIN RESPONSE FACTOR 21, F12K21.26, F12K21_26
    AT1G35240AT1G35240.1 Symbol: ARF20 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g35540.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g34390.1); similar to transcriptional factor B3 family protein [Arabidopsis thaliana] (TAIR:At1g35520.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g34410.1); similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At1g34310.1); similar to ARFO_ARATH Putative auxin response factor 15 (GB:Q9LQE3); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340); contains InterPro domain AUX/IAA protein (InterPro:IPR003311) | chr1:12927435-12930501 REVERSE | Aliases: ARF20, AUXIN RESPONSE FACTOR 20, T9I1.3, T9I1_3
    AT1G35520AT1G35520.1 Symbol: ARF15 | transcriptional factor B3 family protein, contains Pfam profile: PF02362 B3 DNA binding domain | chr1:13082797-13085808 REVERSE | Aliases: ARF15, AUXIN RESPONSE FACTOR 15, F15O4.42
    AT1G35540AT1G35540.1 Symbol: ARF14 | transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related, contains Pfam profiles: PF02362 B3 DNA binding domain, PF02309: AUX/IAA family | chr1:13108612-13111678 FORWARD | Aliases: ARF14, AUXIN RESPONSE FACTOR 14, F15O4.64, F15O4_64
    AT1G59750AT1G59750.1 AT1G59750.2 AT1G59750.3 Symbol: ARF1 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At2g46530.1); similar to OSJNBa0064D20.11 [Oryza sativa (japonica cultivar-group)] (GB:CAE04227.2); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340); contains InterPro domain AUX/IAA protein (InterPro:IPR003311) | chr1:21983067-21988098 FORWARD | Aliases: ARF1, AUXIN RESPONSE FACTOR 1, F23H11.7, F23H11_7
    AT1G77850AT1G77850.1 Symbol: ARF17 | transcriptional factor B3 family protein, similar to auxin response factor 10 GI:6165644 from (Arabidopsis thaliana); contains Pfam profile PF02362: B3 DNA binding domain | chr1:29277207-29280313 FORWARD | Aliases: ARF17, AUXIN RESPONSE FACTOR 17, F28K19.6, F28K19_6
    AT2G28350AT2G28350.1 Symbol: ARF10 | auxin-responsive factor (ARF10), similar to auxin response factor 10 GI:6165644 from (Arabidopsis thaliana); identical to cDNA auxin response factor 10 (ARF10) mRNA, partial cds GI:6165643 | chr2:12121408-12123914 FORWARD | Aliases: ARF10, AUXIN RESPONSE FACTOR 10, T1B3.13, T1B3_13
    AT2G33860AT2G33860.1 Symbol: ETT | auxin-responsive factor (ARF3) / ETTIN protein (ETT), identical to ETTIN GB:AF007788 from (Arabidopsis thaliana) | chr2:14332345-14335847 REVERSE | Aliases: ARF3, AUXIN RESPONSE TRANSCRIPTION FACTOR 3, ETTIN, T1B8.30, T1B8_30
    AT2G46530AT2G46530.1 AT2G46530.2 AT2G46530.3 Symbol: ARF11 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At3g61830.1); similar to auxin-responsive factor (ARF9) [Arabidopsis thaliana] (TAIR:At4g23980.1); similar to OSJNBa0064D20.11 [Oryza sativa (japonica cultivar-group)] (GB:CAE04227.2); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340); contains InterPro domain AUX/IAA protein (InterPro:IPR003311) | chr2:19112184-19115403 FORWARD | Aliases: AUXIN RESPONSE FACTOR 11, F11C10.34
    AT3G61830AT3G61830.1 Symbol: ARF18 | transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related, contains Pfam profile: PF02309 AUX/IAA family | chr3:22898864-22902410 FORWARD | Aliases: ARF18, AUXIN RESPONSE FACTOR 18, F21F14.12
    AT4G23980AT4G23980.1 AT4G23980.2 Symbol: ARF9 | similar to transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related [Arabidopsis thaliana] (TAIR:At2g46530.1); similar to ARFO_ARATH Putative auxin response factor 15 (GB:Q9LQE3); contains InterPro domain Transcriptional factor B3 (InterPro:IPR003340); contains InterPro domain AUX/IAA protein (InterPro:IPR003311) | chr4:12451208-12455277 FORWARD | Aliases: AUXIN RESPONSE FACTOR 9, T32A16.150, T32A16_150
    AT4G30080AT4G30080.1 Symbol: ARF16 | transcriptional factor B3 family protein, contains Pfam profile: PF02362 B3 DNA binding domain | chr4:14703233-14706342 REVERSE | Aliases: ARF16, AUXIN RESPONSE FACTOR 16, F6G3.110, F6G3_110
    AT5G20730AT5G20730.1 AT5G20730.2 AT5G20730.3 Symbol: NPH4 | auxin-responsive factor (ARF7), identical to auxin response factor 7 GI:4104929 from (Arabidopsis thaliana) | chr5:7016472-7022115 REVERSE | Aliases: ARF7, AUXIN RESPONSE FACTOR 7, AUXIN REGULATED TRANSCRIPTIONAL ACTIVATOR 7, AUXIN RESPONSIVE TRANSCRIPTIONAL ACTIVATOR 7, BIP, BIPOSTO, IAA21, MASSUGU1, MSG1, NON PHOTOTROPHIC HYPOCOTYL, T1M15.130, T1M15_130, TIR5, TRANSPORT INHIBITOR RESPONSE 5
    AT5G37020AT5G37020.1 Symbol: ARF8 | auxin-responsive factor (ARF8), identical to auxin response factor 8 GI:4104931 from (Arabidopsis thaliana) | chr5:14647258-14651617 FORWARD | Aliases: AUXIN RESPONSE FACTOR 8, K15O15.1, K15O15_1
    AT5G60450AT5G60450.1 Symbol: ARF4 | auxin-responsive factor (ARF4), contains Pfam profile: PF02362 B3 DNA binding domain; identical to cDNA auxin response factor 4 (ARF4) GI:4102597 | chr5:24325549-24329924 REVERSE | Aliases: AUXIN RESPONSE FACTOR 4, MUF9.7, MUF9_7
    AT5G62000AT5G62000.1 AT5G62000.2 AT5G62000.3 Symbol: ARF2 | transcriptional factor B3 family protein / auxin-responsive factor, putative (ARF1), contains Pfam profile: PF02362 B3 DNA binding domain; identical to cDNA ARF1 (auxin response factor) binding protein GI:2245393 | chr5:24927608-24932431 FORWARD | Aliases: ARF1 BINDING PROTEIN, ARF1 BP, AT5G62010, AUXIN RESPONSE FACTOR 2, HLS1 SUPPRESSOR, HSS, MTG10.1, MTG10_1
    ATMG00940ATMG00940.1 Symbol: ORF164 | hypothetical protein | chrM:251403-251897 REVERSE | Aliases: ORF164

    ARF Family Introduction

    Auxin-response factors (ARFs) bind with specificity to TGTCTC auxin-response elements(AuxREs), which are found in promoters of primary/early auxin-response genes. The ARF1 protein contains an amino-terminal DNA-binding domain (DBD), which has some sequence similarity to a carboxyl-terminal B3 domain found in the maize transcription factor VIVIPAROUS 1 (VP1) and its relatives. ARF1 contains a carboxyl-terminal domain (CTD) related to motifs III and IV found in the CTDs of Aux/IAA proteins, which are short-lived nuclear proteins encoded by a family of primary/early auxin-response genes. The CTDs in ARF1 and Aux/IAA proteins facilitate dimerization among the members of both ARF and Aux/IAA protein families. Transcriptional activation or repression domains in ARFs have not been identified in previous studies.

    Here, we show that some ARFs contain middle regions (i.e., regions separating the DBD from the CTD) that function as activation domains. Our results suggest that TGTCTC AuxREs are occupied regardless of the auxin status in cells and that auxin is unlikely to directly affect the targeting of ARFs to their DNA-binding sites. These prebound transcription factors facilitate activated transcription when auxin levels are elevated or when ARF activators are overexpressed in transfected protoplasts. Our results also suggest that ARF CTDs facilitate interactions between ARF activators that are not bound to DNA and transcription factors (e.g., ARFs) that are bound to AuxREs to further enhance transcription.

    Ulmasov T, Hagen G, Guilfoyle TJ.
    Activation and repression of transcription by auxin-response factors
    Proc Natl Acad Sci U S A. 1999 May 11; 96 (10): 5844¨C5849
    PMID: 10318972

    ARF Family Binding Site

    FamilySubfamily or DomainCis-element nameBinding siteReference
    ARF  R10011 
    ARF  R10013 
    ARF  AuxREsTGTCTCUlmasov T, Proc Natl Acad Sci U S A. 1999;96(10):5844-9 Tim Ulmasov,Science 276(1997)

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    last updated:2006-07-20